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- Order number: 7TM0130A
- Content: 100 µl
- Host: Rabbit
Threonine315 (T315) is major phosphorylation site of the GPR139 Receptor (GPR139). The pT315-GPR139 antibody detects phosphorylation in response to agonists. T315 phosphorylation is likely to be involved in efficient ligand sequestration.
| Alternative Names | GPRG1, G-protein-coupled receptor PGR3 |
| IUPHAR Target ID | 130 |
| Uniprot ID | Q6DWJ6 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from human GPR139 around the phosphorylation site of Thr315. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Threonine315 phosphorylation of the GPR139 Receptor. Upper panel, HEK293 cells stably expressing the GPR139 Receptor (GPR139) and transiently GRK2 (Ratio GPR139 receptor:GRK2 = 10:1) were preincubated with serum-free media. After 2 hours cells were either not exposed or exposed to 100 nM JNJ-63533054 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT315-GPR139 antibody (7TM0130A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-GPR139 antibody to confirm equal loading of the gel.



