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pS362/pS363/pS364-mS1P5 (phospho-mouse-Sphingosine 1-Phosphate Receptor 5 Antibody)

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Agonist-induced Serine362/Serine363/Serine364 phosphorylation of the mouse Sphingosine 1-Phosphate Receptor 5
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  • 7TM0279E
  • 100 µl
  • Rabbit
Serine362/Serine363/Serine364 (S362/S363/S364) is major phosphorylation site of the mouse... more

Serine362/Serine363/Serine364 (S362/S363/S364) is major phosphorylation site of the mouse Sphingosine 1-Phosphate Receptor 5 (mS1P5). The pS362/pS363/pS364-mS1P5 antibody detects phosphorylation in response to agonists. S362/S363/S364 phosphorylation is likely to be involved in efficient ligand sequestration by mS1P5.

  Alternative Names  Edg8, endothelial differentiation G protein-coupled... more

 

Alternative Names  Edg8, endothelial differentiation G protein-coupled receptor 8, NRG-1
IUPHAR Target ID  279
UniProt ID  Q91X56
Western Blot (WB)  1:1000
Species Reactivity   Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic phosphopeptide derived from mouse S1P5 around the phosphorylation site of Ser362/Ser363/Ser364.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Agonist-induced Serine362/Serine363/Serine364 phosphorylation of the mouse... more

Figure 1. Agonist-induced Serine362/Serine363/Serine364 phosphorylation of the mouse Sphingosine 1-Phosphate Receptor 5. Upper panel, HEK293 cells stably expressing the mouse S1P5-Receptor (mS1P5) were either not exposed or exposed to 1 µM of S1P5-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS362/pS363/pS364-mS1P5 antibody (7TM00279E) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-mS1P5 antibody (7TM0279MN) to confirm equal loading of the gel.

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1... more

Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.

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