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- Order number: 7TM0279E
- Content: 100 µl
- Host: Rabbit
Serine362/Serine363/Serine364 (S362/S363/S364) is major phosphorylation site of the mouse Sphingosine 1-Phosphate Receptor 5 (mS1P5). The pS362/pS363/pS364-mS1P5 antibody detects phosphorylation in response to agonists. S362/S363/S364 phosphorylation is likely to be involved in efficient ligand sequestration by mS1P5.
| Alternative Names | Edg8, endothelial differentiation G protein-coupled receptor 8, NRG-1 |
| IUPHAR Target ID | 279 |
| UniProt ID | Q91X56 |
| Western Blot (WB) | 1:1000 |
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic phosphopeptide derived from mouse S1P5 around the phosphorylation site of Ser362/Ser363/Ser364. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Agonist-induced Serine362/Serine363/Serine364 phosphorylation of the mouse Sphingosine 1-Phosphate Receptor 5. Upper panel, HEK293 cells stably expressing the mouse S1P5-Receptor (mS1P5) were either not exposed or exposed to 1 µM of S1P5-Receptor agonists S1P for 30 minutes. Cells were lysed and immunoblotted with the anti-pS362/pS363/pS364-mS1P5 antibody (7TM00279E) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with the phosphorylation-independent anti-mS1P5 antibody (7TM0279MN) to confirm equal loading of the gel.
Be the first to analyze the S1P1 phosphorylation using our anti-pS351/pS353-S1P1 antibody and let us know.



