DP1 (IHC-grade), DP1 Prostanoid Receptor Antibody

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Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney

Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas.

Preview: Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma

Preview: Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells

Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney

Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas.

Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma

Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells

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Order number: 7TM0338N-IC
Content: 100 µl
Host: Rabbit

The non-phospho-DP1 Receptor Antibody is directed against the distal end of the... more

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The non-phospho-DP1 Receptor Antibody is directed against the distal end of the carboxyl-terminal tail of human DP1. It can be used to detect total DP1 receptors in Western blots independent of phosphorylation. The non-phospho-DP1 antibody can also be used to isolate and enrich DP1 receptors from cell and tissue lysates. It also detects DP1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names DP1, prostanoid DP receptor-like, PTGDR1, prostaglandin... more

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Alternative Names	DP1, prostanoid DP receptor-like, PTGDR1, prostaglandin D2 receptor (DP)
IUPHAR Target ID	388
UniProt ID	Q13258
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents carboxyl-terminal tail of human DP1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney. Sections... more

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Figure 1. Immunohistochemical identification of DP1 Prostanoid receptor 1 in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 2. Immunohistochemical identification of DP1 Prostanoid receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 3. Immunohistochemical identification of DP1 Prostanoid receptor 1 in renal cell carcinoma. Sections were dewaxed, microwaved in citric acid, and incubated with anti-DP1 (non-phospho-DP1 Prostanoid Receptor) antibody (7TM0388N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

Figure 4. Validation of the DP1 Prostanoid Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the DP1 prostanoid receptor 1 (DP1) were lysed and immunoblotted according to 7TM Western Blot Protocol with the phosphorylation-independent anti-DP1 antibody (7TM0388N-IC) at a dilution of 1:1000.