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- Order number: 7TM0110N
- Content: 100 µl
- Host: Rabbit
The non-phospho-GPR61 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human GPR61. It can be used to detect total GPR61 receptors in Western blots independent of phosphorylation. The GPR61 antibody can also be used to isolate and enrich GPR61 receptors from tissue lysates. It also detects GPR61 in cultured cells and tissue sections by immunohistochemistry.
| Alternative Names | GPCR3 | BALGR | biogenic amine receptor-like GPCR |
| IUPHAR Target ID | 110 |
| Uniprot ID | Q9BZJ8 |
| Western Blot (WB) | 1:1000 |
| Immunohistochemistry (IHC) | 1:100 |
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Immunogen | A synthetic peptide corresponding to residues distal end of carboxy-terminal tail of human GPR61. |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage buffer | Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide |
| Storage conditions | short-term 4°C, long-term -20°C |
Figure 1. Validation of the GPR61 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR61 Receptor (GPR61) were lysed and immunoblotted with the phosphorylation-independent anti-GPR61 antibody (7TM0110N) at a dilution of 1:1000.
Figure 2. Immunohistochemical identification of G Protein-coupled Receptor 61 in cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR61 (G Protein-coupled Receptor 61) antibody (7TM0110N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution.Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.
Figure 3. Immunohistochemical varification of GPR61 Receptor antibody in pancreas. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR61 (non-phospho-GPR61 Receptor)) antibody (7TM0110N-IC). Sections were then incubated with anti-GPR61 (non-phospho-GPR61 Receptor) antibody (7TM0110N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR61 receptors were uniformly detected at the plasma membrane of cells.
Figure 4. Immunohistochemical varification of GPR61 Receptor antibody in hepatocytes. Sections were dewaxed, microwaved in citric acid, and not exposed (left pannel) or exposed to peptide (right pannel) that was used for production of anti-GPR61 (non-phospho-GPR61 Receptor)) antibody (7TM0110N-IC). Sections were then incubated with anti-GPR61 (non-phospho-GPR61 Receptor) antibody (7TM0110N-IC) at a dilution of 1:100 and sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin. Note, only in sections without peptide incubation GPR61 receptors were uniformly detected at the plasma membrane of cells.