OX1 (IHC-grade) Orexin Receptor 1 Antibody

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Immunohistochemical identification of Orexin Receptor 1 in pancreas

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Delivery time appr. 5-8 days

Order number: 7TM0321N-IC
Content: 100 µl
Host: Rabbit

The non-phospho OX1 receptor antibody is directed against the distal end of the... more

The non-phospho OX1 receptor antibody is directed against the distal end of the carboxyl-terminal tail human OX1 receptor. It can be used to detect total OX1 receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich OX1 receptors from tissue lysates. It also detects OX1 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names OX1R, Hypocretin Receptor 1 IUPHAR Target ID... more

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Alternative Names	OX1R, Hypocretin Receptor 1
IUPHAR Target ID	321
UniProt ID	O43613
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human OX1.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification of Orexin Receptor 1 in pancreas. Sections... more

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Figure 1. Immunohistochemical identification of Orexin Receptor 1 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-OX1 (non-phospho-Orexin Receptor 1) antibody (7TM0321N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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