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GLP2 (non-phospho) Glucagon-Like Peptide 2 Receptor Antibody

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  • 7TM0250N
  • 100 µl
  • Rabbit
The non-phospho GLP2 receptor antibody is directed against the distal end of the... more

The non-phospho GLP2 receptor antibody is directed against the distal end of the carboxyl-terminal tail human GLP2 receptor. It can be used to detect total GLP2 receptors  in Western blots independent of phosphorylation. It can also be used to isolate and enrich GLP2 receptors from tissue lysates. It also detects GLP2 in cultured cells and tissue sections by immunohistochemistry.

  Alternative Names GLP-2R IUPHAR Target ID   250... more

 

Alternative Names GLP-2R
IUPHAR Target ID  250
UniProt ID  O958383
Western Blot (WB)  1:1000
Immunohistochemistry (IHC) 1:100
Species Reactivity   Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Immunogen A synthetic peptide corresponding to c-terminal end of human GLP2.
Form  Liquid
Purification Antigen affinity chromatography
Storage buffer Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions short-term 4°C, long-term -20°C
Figure 1. Validation of the Glucagon-Like Peptide 2 Receptor in transfected HEK293 cells.... more

Figure 1. Validation of the Glucagon-Like Peptide 2 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Glucagon-Like Peptide 2 Receptor (GLP2) were lysed and immunoblotted with the anti-GLP2 antibody (7TM0250N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of  Glucagon-Like Peptide 2 Receptor in enteric ganglion. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GLP2 (non-phospho-Glucagon-Like Peptide 2 Receptor) antibody (7TM0250N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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