CCR6 (non-phospho) Chemokine Receptor 6 Antibody

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Preview: Validation of the CC Chemokine Receptor 6 in transfected HEK293 cells

Preview: Immunohistochemical identification of CC Chemokine Receptor 6 in pancreas

Validation of the CC Chemokine Receptor 6 in transfected HEK293 cells

Immunohistochemical identification of CC Chemokine Receptor 6 in pancreas

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Order number: 7TM0063N
Content: 100 µl
Host: Rabbit

The non-phospho-CCR6 receptor antibody is directed against the distal end of the... more

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The non-phospho-CCR6 receptor antibody is directed against the distal end of the carboxyl-terminal tail of human CCR6. It can be used to detect total CCR6 receptors in Western blots independent of phosphorylation. The CCR6 antibody can also be used to isolate and enrich CCR6 receptors from tissue lysates. It also detects CCR6 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names CCR6, CKR-L3, Chemokine Receptor 6, Chemokine (C-C motif)... more

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Alternative Names	CCR6, CKR-L3, Chemokine Receptor 6, Chemokine (C-C motif) Receptor 6, DRY-6
IUPHAR Target ID	63
UniProt ID	P51684
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to c-terminal end of human CCR6.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the CC Chemokine Receptor 6 in transfected HEK293 cells. Native... more

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Figure 1. Validation of the CC Chemokine Receptor 6 in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the CC Chemokine Receptor 6 (CCR6) were lysed and immunoblotted with the anti-CCR6 antibody (7TM0063N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of CC Chemokine Receptor 6 in pancreas. Sections were dewaxed, microwaved in citric acid, and incubated with anti-CCR6 (CC Chemokine Receptor 6) antibody (7TM0063N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK,... more

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Isaikina P, Petrovic I, Jakob RP, Sarma P, Ranjan A, Baruah M, Panwalkar V, Maier T, Shukla AK, Grzesiek S. A key GPCR phosphorylation motif discovered in
arrestin2⋅CCR5 phosphopeptide complexes. Mol Cell. 2023 Jun 15;83(12):2108-2121.e7. doi: 10.1016/j.molcel.2023.05.002. Epub 2023 May 26. PMID: 37244255.

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