SMO (non-phospho) Smoothened Receptor Antibody

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Preview: Validation of the Smoothened Receptor in transfected HEK293 cells

Preview: Immunohistochemical identification of Smoothened Receptor in kidney

Validation of the Smoothened Receptor in transfected HEK293 cells

Immunohistochemical identification of Smoothened Receptor in kidney

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Order number: 7TM0239N
Content: 100 µl
Host: Rabbit

The non-phospho SMO receptor antibody is directed against the distal end of the... more

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The non-phospho SMO receptor antibody is directed against the distal end of the carboxyl-terminal tail human SMO receptor. It can be used to detect total SMO receptors in Western blots independent of phosphorylation. It can also be used to isolate and enrich SMO receptors from tissue lysates. It also detects SMO in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names SMOH | FZD11 | frizzled family... more

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Alternative Names	SMOH \| FZD11 \| frizzled family member 11 \| bnb \| smoothened, frizzled class receptor \| smoothened
IUPHAR Target ID	239
UniProt ID	Q99835
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide presents the carboxyl-terminal tail of human SMO.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Validation of the Smoothened Receptor in transfected HEK293 cells. Native HEK293... more

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Figure 1. Validation of the Smoothened Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the Smoothened Receptor (SMO) were lysed and immunoblotted with the phosphorylation-independent anti-SMO antibody (7TM0239N) at a dilution of 1:1000.

Figure 2. Immunohistochemical identification of Smoothened Receptor in kidney. Sections were dewaxed, microwaved in citric acid, and incubated with anti-SMO (non-phospho-Smoothened Receptor 1) antibody (7TM0239N) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-rabbit IgG and avidin-biotin solution. Color was developed by incubation in 3-amino-9-ethylcarbazole (AEC), and sections were counterstained with hematoxylin.

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