GPR3 (IHC-grade), G Protein-Coupled Receptor 3 Antibody

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Preview: Immunohistochemical identification of GPR3 Receptor in Cortex

Preview: Validation of the GPR3 Receptor in transfected HEK293 cells

Immunohistochemical identification of GPR3 Receptor in Cortex

Validation of the GPR3 Receptor in transfected HEK293 cells

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Order number: 7TM0083N-IC
Content: 100 µl
Host: Rabbit

The GPR3 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat... more

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The GPR3 antibody is directed against the distal end of the carboxyl-terminal tail of mouse, rat and human GRP3. It can be used to detect total GPR3 receptors in Western blots independent of phosphorylation. The GPR3 antibody can also be used to isolate and enrich GPR3 receptors from brain lysates. It also detects GPR3 in cultured cells and tissue sections by immunohistochemistry.

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Alternative Names Gpcr21, GPCR3, ACCA orphan receptor IUPHAR Target... more

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Alternative Names	Gpcr21, GPCR3, ACCA orphan receptor
IUPHAR Target ID	83
UniProt ID	P46089
Western Blot (WB)	1:1000
Immunohistochemistry (IHC)	1:100
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic peptide corresponding to distal end of carboxy-terminal tail of human GPR3.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Immunohistochemical identification... more

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Figure 1. Immunohistochemical identification of GPR3 Receptor in Cortex. Sections were dewaxed, microwaved in citric acid, and incubated with anti-GPR3 (GPR3 Receptor) antibody (7TM0083N-IC) at a dilution of 1:100. Sections were then sequentially treated with biotinylated anti-guinea pig IgG and avidin-biotin solution. Sections were then developed in 3,3-diaminobenzidine (DAB)-glucose oxidase and lightly counterstained with hematoxylin.

Figure 2. Validation of the GPR3 Receptor in transfected HEK293 cells. Native HEK293 cells (MOCK) or HEK293 cells stably expressing the GPR3 Receptor (GPR3) were lysed and immunoblotted with the phosphorylation-independent anti-GPR3 antibody (7TM0083N-IC) at a dilution of 1:1000.