pT419/pT421/pT422-RXFP3 (phospho-Relaxin Receptor 3 Antibody)

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Agonist-induced Threonine419/Threonine421/Threonine422 phosphorylation of the Relaxin Receptor 3

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Order number: 7TM0353A
Content: 100 µl
Host: Rabbit

Threonine419/Threonine421/Threonine422 (T419/T421/T422) is major phosphorylation site of... more

Threonine419/Threonine421/Threonine422 (T419/T421/T422) is major phosphorylation site of the Relaxin Receptor 3 (RXFP3). The pT419/pT421/pT422-RXFP3 antibody detects phosphorylation in response to agonists. T419/T421/T422 phosphorylation is likely to be involved in efficient ligand sequestration by Relaxin.

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Alternative Names RLN3R1, GPCR135, relaxin/insulin like family... more

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Alternative Names	RLN3R1, GPCR135, relaxin/insulin like family peptide receptor 3
IUPHAR Target ID	353
UniProt ID	Q9NSD7
Western Blot (WB)	1:1000
Species Reactivity	Human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Immunogen	A synthetic phosphopeptide derived from human RXFP3 around the phosphorylation site of Thr419/Thr421/Thr422.
Form	Liquid
Purification	Antigen affinity chromatography
Storage buffer	Dulbecco's PBS, pH 7.4, with 150 mM NaCl, 0.02% sodium azide
Storage conditions	short-term 4°C, long-term -20°C

Figure 1. Agonist-induced Threonine419/Threonine421/Threonine422 phosphorylation of the Relaxin... more

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Figure 1. Agonist-induced Threonine419/Threonine421/Threonine422 phosphorylation of the Relaxin Receptor 3. Upper panel, HEK293 cells stably expressing the RXFP3-Receptor (RXFP3) were either not exposed or exposed to 1 µM of RXFP3-Receptor agonists Relaxin-3 for 30 minutes. Cells were lysed and immunoblotted with the anti-pT419/pT421/pT422-RXFP3 antibody (7TM00353A) at a dilution of 1:1000. Lower panel, blot was stripped and reprobed with an phosphorylation-independent anti-RXFP3 antibody to confirm equal loading of the gel.

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Download 7TM Western Blot Protocol

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